Table 1.
Permeability ratios (PK+/PCl−) and conductance values (g) for various α-HLs with and without adapters
| Adapter | Charge, e | Minimum diameter, Å | wt-αHL
|
αHL-M113N
|
αHL-E111N/K147N
|
αHL-CH1
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vr, mV | PK+/PCl− | g, pS* | Vr, mV | PK+/PCl− | g, pS* | Vr, mV | PK+/PCl− | g, pS* | Vr, mV | PK+/PCl− | g, pS† | |||
| None | 14 | +9.1‡ | 0.55 ± 0.02 | 658 ± 11 | +5.96‡ | 0.68 ± 0.03 | 622 ± 9 | +2.71§ | 1.2 ± 0.1 | 634 ± 12 | +27.1¶ | 5.1 ± 0.2 | 541 ± 11 | |
| −3.7§ | 0.79 ± 0.02 | −2.21§ | 0.87 ± 0.04 | |||||||||||
| βCD | 0 | 6.2 | +19.6‡ | 0.25 ± 0.01 | 240 ± 5 | +29.7‡ | 0.079 ± 0.005 | 261 ± 4 | −24.9§ | 0.15 ± 0.02 | 207 ± 7 | −3.9¶ | 0.82 ± 0.01 | 109 ± 9 |
| −20.5§ | 0.23 ± 0.01 | −32.3§ | 0.046 ± 0.006 | |||||||||||
| s7βCD | −7 | <6.2 | −24.1‡ | 6.7 ± 0.4 | 53 ± 6 | −19.3‡ | 4.1 ± 0.02 | 40 ± 1 | ND | ND | ND | ND | ND | ND |
| +28.1§ | 10.0 ± 0.2 | +23.2§ | 6.1 ± 0.1 | |||||||||||
| γCD | 0 | 7.9 | −14.0§ | 0.38 ± 0.04 | 328 ± 4 | ND | ND | 287 ± 5 | ND | ND | 275 ± 4 | ND | ND | ND |
For each entry, three or more separate experiments were performed, and data acquired for at least 1 min were analyzed. The reversal potentials (Vr) are mean values under the conditions stated. Permeability ratios are quoted as the mean ± SD. ND, not determined.
*−40 mV, 1 M NaCl, 10 mM sodium phosphate (pH 7.5).
†−40 mV 1 M KCl, 10 mM potassium phosphate (pH 7.4).
‡pH 7.5, KCl in mM (cis/trans) was (200/1000).
§pH 7.5, KCl in mM (cis/trans) was (1000/200).
¶pH 7.4, KCl in mM (cis/trans) was (1000/100).