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. 2003 Aug;132(4):1728–1738. doi: 10.1104/pp.103.024414

Figure 1.

Figure 1.

Time-course analysis of MAPK activity induced by systemin and OEs. L. peruvianum cells were left untreated or were supplied with systemin (3 nm) or the OEs chitin (100 μm chitotetraose), chitosan (1.7 μg mL1), β-glucan (10 μg mL1), and PGA (830 μg mL1). At the times indicated, samples were quick-frozen and assayed for MAPK activity in in-gel kinase assays. Radioactive bands were visualized by phosphoimaging. Arrows with numbers indicate the apparent molecular masses of systemin- and OE-responsive MAPKs.