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. 2003 Aug;132(4):1728–1738. doi: 10.1104/pp.103.024414

Figure 2.

Figure 2.

MAPK activity in response to consecutive OE treatments. L. peruvianum suspension-cultured cells were pretreated with the OEs chitin (100 μm chitotetraose), chitosan (1.7 μg mL–1), β-glucan (10 μg mL–1), or PGA (830 μg mL–1), or were left untreated. At 30, 60, 90, and 120 min thereafter, treated and untreated cells were left untreated or were supplied a second time with the same OE. Ten minutes thereafter, cells were quick-frozen and assayed for MAPK activity. Samples corresponding to a given time point (lanes 1–4) were taken at the same time. Lane 1, Untreated control. Lane 2, MAPK activity in response to the pretreatment. Lane 3, MAPK activity in response to the second treatment without pretreatment. Lane 4, MAPK activity in response to the second treatment after a pretreatment.