Figure 4.

Expression of TaVRT-1 in relation to vernalization, COR gene expression, FLN, and development of FT. RNA gel-blot analyses showing TaVRT-1 transcript accumulation in wheat parental lines spring wheat cv Manitou (a) and winter wheat cv Norstar (b) and in the wheat near-isogenic lines winter wheat cv Manitou (c) and spring wheat cv Norstar (d). Blots were hybridized sequentially with the specific TaVRT-1 probe and the WCS120 and WCS19/LEA3-L2 probes. rRNA is shown as a load control. FLN (—) and LT₅₀ (....) for wheat cv Manitou (e) and wheat cv Norstar (f), and their NILs are also illustrated.