Table I.
Purification of engineered plastid TK from maize
Procedure | Total Activity | Total Protein | Specific Activity | Purification | Yield |
---|---|---|---|---|---|
μmol min-1 | mg | μmol min-1 mg-1 | -fold | % | |
Crude extract | 438 | 73 | 6.0 | 1 | 100 |
Ni-chelating agarose | 388 | 38 | 10.2 | 1.7 | 89 |
Superdex 200 | 380 | 15 | 25.3 | 4.2 | 87 |