Figure 2.
Reducing and denaturing SDS-PAGE of purified antibodies. The 3H9 (lane 1), 1A11 (lane 2), and 4H8 (lane 3) IgG were purified on Protein G-sepharose and examined on 12% PAGE. The mobilities of the IgG H and L chains were determined relative to molecular weight markers (MW). Note absence of contaminating proteins and difference in migration between the L chains of the clonally related IgG.