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. Author manuscript; available in PMC: 2008 Mar 1.
Published in final edited form as: Mol Immunol. 2006 Nov 3;44(8):1914–1921. doi: 10.1016/j.molimm.2006.09.027

Figure 6.

Figure 6

Binding of 4H8, 1A11, and 3H9 to apoptotic blebs and nuclear fragments is unaffected by PNGase F. Jurkat cells, treated with camptothecin to induce apoptosis, were fixed and incubated with 4H8 (A), 1A11 (B), and 3H9 (C). Equivalent reactions were incubated with 4H8 (D), 1A11 (E), and 3H9 (F) treated with PNGase F. Bound antibodies were detected with anti-mouse antibodies (displayed in red). Each of the antibodies, regardless of PNGase F treatment, bound at or near the surface of nuclear fragments. DNA, bound by Sytox Orange, is displayed in blue and annexin V in green. In addition to the composite images, separate wavelength channels are displayed at 1/3 size. Images represent optical cross sections. Controls included cells incubated in the absence of mouse IgG and cells incubated in the presence of z-VAD-fmk, neither of which showed antibody binding (data not shown).