Figure 4.

Analysis of transcript accumulation of VfPTR1, VfPTR2, and VfAAP1 in seedlings of faba bean harvested at different time points. Ten micrograms of total RNA extracted from different parts of the seedlings and at different time points was loaded per lane; i.e. before roots had ruptured the seed coats (4 dai), when roots were between 0.5 and 1.0 cm long (6 dai), and when roots were approximately 4.0 cm long (8 dai). Northern blots were hybridized with ³²P-labeled cDNA probes of VfPTR1 (a), VfPTR2 (b), and VfAAP1 (c). An approximate size scale (kilobytes) is indicated. Equal loading between samples was confirmed by running an ethidium bromide-stained gel (data not shown).