Analysis of transcript accumulation of VfPTR1, VfPTR2,
and VfAAP1 in seedlings of faba bean harvested at different time
points. Ten micrograms of total RNA extracted from different parts of the
seedlings and at different time points was loaded per lane; i.e. before roots
had ruptured the seed coats (4 dai), when roots were between 0.5 and 1.0 cm
long (6 dai), and when roots were approximately 4.0 cm long (8 dai). Northern
blots were hybridized with 32P-labeled cDNA probes of
VfPTR1 (a), VfPTR2 (b), and VfAAP1 (c). An
approximate size scale (kilobytes) is indicated. Equal loading between samples
was confirmed by running an ethidium bromide-stained gel (data not shown).