Transcript distribution of VfPTR1 in sections of developing faba
bean seeds and seedlings. a, Schematic representation of a developing faba
bean embryo in a cross-section, where the boxed areas are enlarged in the
following pictures; b through k, Bright-field micrographs showing in situ
hybridization using a 33P-labeled VfPTR1 probe, with label
seen as dark grains; b, abaxial region of a stage IV
(Borisjuk et al., 1995)
cotyledon with labeled epidermal cells and endosperm, but unlabeled parenchyma
cells; c and d, abaxial and adaxial regions of cotyledons, respectively. The
abaxial transfer cells in the outer epidermis are more strongly labeled than
the cells of the inner epidermis; e, labeled cells surrounding transport
vessels of a stage VI cotyledon; f, strong labeling of endosperm surrounding
the axis during stage VI; g, cotyledons with labeled storage parenchyma cells
and transfer cells of the outer epidermal region; h, radicle of a stage VII
embryo strongly labeled within the cortex cells, but not the epidermis cells
(seen as an unlabeled layer between the cortex and the endosperm). i through
k, Distribution of VfPTR1 transcripts in sections of faba bean
seedlings; i, section of a root showing the labeled vascular bundles; j,
longitudinal section through the seedling, showing strong label in the
hypocotyl and in the epidermis of the root, but not in the epidermis of the
hypocotyl; k, cross-section through the root of a seedling showing labeling
within the epidermis and the root hairs; ab, Abaxial region; ad, adaxial
region; ax, axis; co, cotyledons; e, endosperm; ep, epidermis; ep(h),
epidermis of hypocotyl; ep(r), epidermis of root; hy, hypocotyl; rc, radicle
cortex; rh, root hair; v, vascular bundle. Bars in b, c, d, e, f, i, and k =
0.1 mm; g and j = 1 mm; h = 0.5 mm. Arrows point to the labeling and
arrowheads point to unlabeled areas.