Figure 5.

Overexpression of BWMK1 in transgenic tobacco plant induces HR-like cell death and elevates SAR gene expression. A, Development of HR-like cell death in transgenic tobacco plants. Lesions formed in 6-week-old representative transgenic BWMK1 tobacco plants (4-11 and 6-2) and wild-type (WT) plant. B, Accumulation of autofluorescent materials in HR-like cell death lesions of transgenic plants. Fully expanded leaves from 6-week-old plants were used for microscopic analysis after clearing with lactophenol. Magnification is ×50. White, Lactophenol-cleaned leaves examined under a light microscope. UV, UV-stimulated autofluorescence in lactophenol-cleared tobacco leaves. C, Constitutive expression of PR genes in transgenic plants.