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In situ hybridization analysis for PCNA and cyclin B transcripts in the apical meristematic region of E2Fa-DPa plants. A and B, Detection with digoxygenin (DIG)-labeled antisense PCNA RNA. C and D, Detection with DIG-labeled antisense RNA for cyc29, a B-type cyclin gene from tobacco. A and C, Cross-section of the apical meristematic region of a 35S-GUS tobacco plant as a control. B and D, Cross-section of a 35S-E2Fa-DPa transgenic line, H-F3D.
