Figure 5.

Altered phenotypes of mesophyll cells of E2Fa-DPa plants. A through C, Slices of mature leaves observed under white light. Pieces cut from mature leaves (about 12 cm in length) were sliced into 60-μm-thick sections. The leaves were from corresponding positions of plants with 14 to 18 leaves. D through F, Nuclei of mature leaves. The same slices, stained with 4′,6-diamidino-2-phenylindole (DAPI), were observed with an epifluorescent microscope. G through I, Nuclei of immature leaf sections stained with DAPI. A, D, and G, 35S-GUS tobacco plant as a control. B, E, and H, 35S-E2Fa-DPa transgenic line, H-F3D. C, F, and I, 35S-E2Fa-DPa transgenic line, L-H3D. Note that in the E2Fa-DPa plants, there was a decrease in cell size and increases in cell number and nuclear size (in H-F3D), and increased numbers of idioblastic cells.