Figure 6.

DNA content and ploidy level. A, DNA content of mature leaves. Total DNA was isolated from mature leaves (200 mg) of the indicated transgenic plants, 35S-GUS (control), and 35S-DPa, -E2Fa, and -E2Fa-DPa/H-F3D. DNA content was calculated as micrograms relative to milligrams of protein isolated from the same leaf. Bars represent mean values of triplicate data. B, Flow cytometric analysis of nuclear DNA content of mature leaves. Nuclei from the indicated transgenic plants were stained with DAPI and the degree of fluorescence was represented as DNA content relative to the main peak of 35S-GUS plants (control) given a ploidy level of 2C.