Figure 1.

RT-PCR analysis of the transcript expression of the Arabidopsis Arp2/3 subunit genes in various tissues. RNA was isolated from organs of Arabidopsis COL-0 WT plants grown in light and used for RT-PCR analysis as described in the text. A 25-cycle PCR reaction was carried out for all genes except for ARPC4/p20, for which 30 cycles were used. ACTIN2 was used as a control except for pollen RT-PCR that involves the use of the ACTIN3 gene.