Characterization of the Arp2/3 subunit mutants. A, The location of the
T-DNA insertions in arp2-1, arp2-2,
arp3-1, and arpc5-1/p16-1 are shown on the
maps of AtARP2, AtARP3, and AtARPC5/p16 genes.
Arrows indicate the location of primers used for RT-PCR analysis of
transcripts. Exons are boxed and introns and untranscribed flanking sequences
are shown as lines. B, RT-PCR analysis of transcript levels for
arp2-1, arp2-2, arp3-1, and
arpc5/p16-1 mutants. Total RNA was extracted from
inflorescences of each homozygous mutant plant and WT plants. The full-length
coding sequence was amplified for each primer set.