Table I.
Purification of Zea m 1 from maize pollen
Twenty grams of maize pollen was extracted, centrifuged, and filtered to give the crude extract. This extract was then subjected to conventional chromatography on CM-Sepharose followed by HPLC on CM-silica-based matrix. This purification was repeated two additional times with similar results.
| Purification Step | Protein | Activity | Specific Activity | Purification | Protein Yield |
|---|---|---|---|---|---|
| mg | % h-1 | % h-1mg-1protein | -fold | % | |
| Crude extract | 580.4 | 2,868 | 4.9 | 1 | 100 |
| CM-Sepharose | 35.1 | 3,212 | 91.5 | 19 | 6.0 |
| CM-HPLC | |||||
| Zea m 1a | 3.0 | 410 | 136.7 | 28 | 0.5 |
| Zea m 1b | 3.4 | 429 | 126.2 | 26 | 0.6 |
| Zea m 1c | 5.1 | 663 | 130.0 | 27 | 0.9 |
| Zea m 1d | 8.6 | 1205 | 140.1 | 29 | 1.5 |