Table I.
Peak emission temperatures (TM) and contribution of different glow peaks to the total thermoluminescence in control (non-hardened) and cold-acclimated Arabidopsis leaves
The contribution of glow peaks represented by characteristic Gaussian sub-bands was estimated by a nonlinear least-squares fitting of the experimental glow curves obtained after illumination with two single turnover flashes. The data are represented as a percentage of the total area (A, %). Mean values ± se were calculated from three to five independent experiments. n.d., Non-detectable. The measurements were performed in the absence and presence of 20 μ m DCMU.
| Sample
|
Control
|
+DCMU
|
||
|---|---|---|---|---|
| Tm | A | Tm | A | |
| °C | % | °C | % | |
| Non-hardened | -12.0±0.06 | 5.1±0.1 | -17.6±0.08 | 7.9±0.1 |
| 37.7±0.03 | 67.8±0.1 | n.d. | n.d. | |
| 53.9±0.06 | 27.1±0.1 | 58.7±0.08 | 92.1±0.2 | |
| Cold acclimated | 0.3±0.1 | 9.1±0.1 | -8.1±0.02 | 25.5±0.1 |
| 28.3±0.02 | 45.6±0.1 | n.d. | n.d. | |
| 48.6±0.06 | 45.3±0.2 | 55.3±0.07 | 74.5±0.2 | |