HPLC analysis of the prenyl quinones from wild-type and mutant Arabidopsis,
maize, and Synechocystis sp. PCC6803. Lipids were extracted from
Arabidopsis, maize, and Synechocystis sp. PCC6803, and total prenyl
quinines were isolated by thin-layer chromatography (TLC) and then analyzed by
normal phase HPLC (see “Materials and Methods”) A, Arabidopsis.
Solid line, Columbia wild type; dotted line, vte1-1; gray line,
vte1-2. B, Maize. Solid line, Wild type; dotted line, sxd1.
C, Synechocystis sp. PCC6803. Solid line, Wild type; dotted line,
Δslr1737 insertional mutant; gray line, SXD1cDNA expressed in
the Δslr1737 mutant background. Insets, Spectra of the peak
labeled DMPBQ. Phyllo, Phylloquinone; PQ, Plastoquinone.