The effect of Al (0–1,000 μm) on WT yeast (MMYO11). A,
Reduction in growth of WT grown in LPP medium for 20 h, with different
concentrations of Al. Vertical bars represent se (n = 3).
B, Transcript levels of CIT1, ACO1, and IDH1 in WT yeast.
Total RNA isolated from yeast cells exposed to different concentrations of Al
for 20 h was probed with 32P-labeled fragments of CIT1,
ACO1, and IDH1. C, Relative abundance of CIT1, ACO1,
and IDH1 transcripts based on densitometry quantification of the
RNA.