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. 2003 Aug;132(4):2205–2217. doi: 10.1104/pp.103.023903

Figure 7.

Figure 7.

Construction of transgenic plants overexpressing CS. Details of the construct (A), genomic DNA PCR analysis (B) used in transformation of canola with At-mtCS. A, Diagrammatic representation of pACS121-Hm. The gene, At-mtCS, is expressed under the control of a constitutive 35S promoter. The construct has selectable markers for NPT II and HPT II genes. The two primer pairs used in genomic DNA PCR amplification are indicated as arrows in the construct. B, Genomic DNA PCR analysis of transformants confirming the presence of At-mtCS transgene. Genomic DNA was isolated from WT and two independent transgenic lines (CS1 and CS12) and was used as template for PCR with two primer pairs indicated above. 35S-F/AtCS-R, Expected amplification size of 0.7 kb; 35S/HPT II-R, expected amplification sizes of 0.3 and 2.8 kb. Positive (+) control includes plasmid DNA (pACS121-Hm), and negative (-) control includes PCR reactions with no DNA.