Table I.
Yeast strains defective in various TCA and glyoxylate cycle genes used in this study are listed below
The genetic background of all mutants listed below is same as the WT, MMYO11 (Przybyla-Zawislak et al., 1999).
| Strain | Genotype | Properties |
|---|---|---|
| MMYO11 | Matα ade2-1 can1-100 his3-11, 15 leu2-3, 112 trp1-1 ura3-1 | Wild-type parental strain |
| Δcit1 | cit1::LEU2 | Defective in mitochondrial citrate synthase CIT1 |
| Δcit2 | cit2::URA3 | Defective in glyoxysomal citrate synthase CIT2 |
| Δcit3 | cit3::URA3 | Defective in mitochondrial citrate synthase CIT3 |
| Δcit12 | cit1:: LEU2 cit2::URA3 | Defective in CIT1 and CIT2 |
| Δcit13 | cit1:: LEU2 cit1::URA3 | Defective in CIT1 and CIT3 |
| Δcit23 | cit2:: URA3 cit3::URA3 | Defective in CIT2 and CIT3 |
| Δcit123 | cit1:: LEU2 cit2::URA3 cit3::URA3 | Defective in CIT1, CIT2, and CIT3 |
| Δaco1 | aco1::URA3 | Defective in mitochondrial aconitase ACO1 |
| Δidh12 | idh1::URA3 idh2::HIS3 | Defective in subunits 1 and 2 of mitochondrial isocitrate dehydrogenase, IDH1 and IDH2 |