Figure 3.

Induction of the PRLIP1 and PRLIP2 genes in Arabidopsis accessions WS and Col-0 in response to BABA and BTH treatments. A, Four-week-old soil-grown plants were treated with BABA (300 μm) or BTH (300 μm) as soil drench. The plants were harvested 1 to 4 d post treatment, as indicated at the top of the figure. RNA blots were hybridized with the probes indicated on the right of the figure. The membranes were exposed to Biomax films (Eastman Kodak, Rochester, NY) for 12 h (PRLIP1, PRLIP3, and PR1) or for 48 h (PRLIP2). B, The specificity of the PRLIP probes was tested by slot blot of the cloned cDNA fragments. Plasmid DNA (1 ng of each) containing a fragment of the different PRLIP cDNAs, as indicated under the figure, was loaded in replicates. The blots were hybridized with the probes indicated on the right of the figure. DNA of the plasmid pUC19 (1 ng) was used as negative control.