Figure 9.

phyC Contributes Marginally to the Rc Repression of ATHB-2 Expression.

(A) RNA gel blot of RNA extracts from wild-type Col-0 and phyC-2 after 4 days plus 6 h of growth in the dark (D6h lanes) or after 4 days of growth in the dark followed by 6 h of Rc irradiation (7 μmol·m⁻²·s⁻¹; Rc6h lanes). As a control, the blot was reprobed to detect 18S RNA.

(B) Histogram of the relative expression levels of ATHB-2 mRNA in wild-type Col-0 and phyC-2 normalized to 18S rRNA levels. Mean values and standard errors (bars) were obtained by quantification of triplicate RNA gel blots (including the one shown in [A]), prepared from three separate seedling batches.