Table 2.
List of the PCR-Based Molecular Markers Developed in This Study
| Marker | Primer Pairsa | Fragment Sizeb | Restriction Enzyme |
|---|---|---|---|
| 36N13f | F, 5′-AATCTTCTCTTACTCCACTCGC-3′; R, 5′-ATGGGAACTACTGACTAAACCG-3′ | C = 489, b = 0 | |
| 57M10r | F, 5′-AGGAACAGATGGAATCTCAGG-3′; R, 5′-TGTCTCCTGTCCCAAACTAGC-3′ | C = 340, b = 0 | |
| 52G19f | F, 5′-GCACGCATTTAAGAAGCAGG-3′; R, 5′-TTATATGACCGTATGGCAGG-3′ | 502 | MnlI |
| P1 | F, 5′-TTGCGTATGTCTGTCAACTGC-3′; R, 5′-TTCCAACATCTGAACCCTCG-3′ | C = 1580, b = 0 | |
| P2 | F, 5′-GTTGCTCATCGTCACCATCG-3′; R, 5′-AGGTATAGAGCGAGCGGTAGC-3′ | 1037 | NsiI |
| P3 | F, 5′-GGTAGTTGAAGTTTGTGATGGC-3′; R, 5′-ATGCTCTCTCCTCGCTCTGC-3′ | 1110 | BstNI |
| P4 | F, 5′-TACTACAACGACCTGCTTATGG-3′ R, 5′-TGGAGAGCAGTGTAGGTAGGG-3′ | 828 | FokI |
| P5 | F, 5′-AGCAGCAGTAGTCGCAGTCG-3′; R, 5′-AACATAGCCATCTGGGGTCC-3′ | 1762 | MnlI |
| P6 | F, 5′-TAGAAACCAGGGTCAAGTAGGC-3′; R, 5′-GCTGAGTTTGAGGTCGGTGG-3′ | 1470 | MseI |
| P7 | F, 5′-AGTGCCTCATCTATCGCTTCG-3′; R, 5′-GTTCCACAAACCGATCTAGCG-3′ | 2046 | ScaI |
a
F, forward primer; R, reverse primer.
b
Numbers indicate the size (in bp) of amplified fragments. b, bc1-2 mutant; C, C-Bao.