Figure 1.
UV-B Light Sensitivities of Wild-Type and rev3-1 Seedlings under Light and Dark Conditions.
(A) Root growth of wild-type (Columbia [Col]) and rev3-1 seedlings in the light condition. Three-day-old seedlings were exposed to 3 kJ m−2 UV-B light and then incubated under continuous white light for 3 days.
(B) Root growth of wild-type and rev3-1 seedlings in the dark condition. Seedlings were exposed to 0.6 kJ m−2 UV-B light and then incubated in the dark for 3 days.
(C) and (D) UV light dose-response curve for root growth in the wild type (open circles) and rev3-1 (closed circles) under light (C) and dark (D) conditions. Root growth after irradiation was measured using NIH Image. Each value represents an average of 18 to 26 measurements. Error bars indicate sd.
(E) Analysis of root growth rate after UV irradiation. Wild-type and rev3-1 seedlings were sown on a nutritive agar plate, and root growth was checked every day. Three days after the start of incubation, the seedlings were exposed to 2 kJ m−2 UV-B light (arrow). Each value represents an average of 15 to 21 measurements. Error bars indicate sd.