Table 1.

Effect of Metabolic Inhibitors on Tubule Formation

Inhibitor a	Percent of Tubules b	Percent of Abnormal Tubules c	b/c d × 100
None	72	0	0
Latrunculin B (2 μM)	77	0	0
Cytochalasin D (20 μM)	66	0	0
Taxol (2 μM)	65	0	0
Oryzalin (10 μM)	74	35	47
Oryzalin (10 μM) e	70	70	100
BFA (10 μg/mL)	14	0	0

^aMetabolic inhibitors were added 8 h after induction at the indicated final concentrations.

^bPercentage of fluorescent BY-2 cells that formed tubules. At least 100 fluorescent cells were observed in each case. Unless indicated otherwise, observations were made 24 h after induction (16 h after the addition of inhibitors).

^cPercentage of fluorescent cells that formed tubules on side walls and cross walls.

^db/c relates to percentage of fluorescent BY-2 cells that form tubules (b) divided by the percentage of fluorescent cells that formed tubules on side walls and cross walls (c).

^eObserved 48 h after induction (40 h after the addition of inhibitors).