Figure 1.

Proteins of the IMS and OMM Separated by Two-Dimensional Gel Electrophoresis.

An IMS/OMM fraction was prepared from isolated Arabidopsis mitochondria by osmotic shock and centrifugation (for details, see Methods). Proteins were separated by isoelectric focusing on 3 to 10 nonlinear immobilized pH gradients for the first dimension and by SDS-PAGE for the second dimension. For clarity, only the relevant section of the gel is shown. The numbered spots are those that have been identified by MS/MS (see Table 2).