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Submitochondrial Localization of Aldolase Is Revealed by a Protease Protection Assay.
The protease thermolysin was added to isolated mitochondria in the presence or absence of Triton X-100 to rupture the mitochondrial membranes. The reaction was terminated by the addition of EGTA, and 50 μg of the protein sample was fractionated by SDS-PAGE before being transferred to a nitrocellulose membrane. The membrane was probed with antisera against aldolase and fumarase.
