Table 2.

Identification of Glycolytic Proteins in an IMS/OMM Fraction of Arabidopsis Mitochondria

Enzyme	Spot No.	AGI Number	No. MP (P<0.05)	Match MM/pI	Gel MM/pI
Aldolase	1	At3g52930	8	38,858/6.05	40,000/6.8
Glyceraldehyde-3-P dehydrogenase	2	At3g04120	3	37,077/6.34	40,000/7.3
Phosphoglycerate mutase	3	At1g09780	4	60,857/5.32	66,000/5.7
Enolase	4	At2g36530	13	47,947/5.54	57,000/6.0

Spot number refers to the protein spot excised from the gel shown in Figure 1. AGI number is the locus identifier given by the Arabidopsis Genome Initiative. No. MP refers to the number of peptides matching the same protein (at P < 0.05). Match MM/pI indicates the predicted molecular mass/pI of the translated open reading frame. Gel MM/pI indicates the molecular mass/pI observed on the gel shown in Figure 1. IMS/OMM was isolated from isolated Arabidopsis mitochondria by osmotic shock and centrifugation. A total of 500 μg of IMS/OMM protein was fractionated by isoelectric focusing and SDS-PAGE. The indicated protein spots were excised from the gel, digested with trypsin, and analyzed by MS/MS. Spectra obtained after collision-induced dissociation were matched to translated Arabidopsis open reading frames.