Characteristics of Retransformed Plants.
(A) Phosphorimage of the hybridization of XhoI-digested genomic DNA probed with GUS. The positions of the restriction sites and expected fragments are indicated as in Figure 1B. The lanes labeled M contain restriction fragments from pAGCNA as relevant markers. The lanes labeled P contain DNA from parent lines before in planta recombination.
(B) Hybridization of EcoRI-AscI–digested DNA probed with NPTII.
(C) Natural logarithm (ln) of the GUS activity in leaves of individual plants obtained after in vitro regeneration of AGCNA-16 (closed circles) and after retransformation with pEX-creINT (open circles). For line AGCNA-54, GUS activity in regenerated (closed squares) and retransformed (open squares) plants is given. For AGCNA-61, GUS activity in regenerated (closed diamonds) and retransformed (open diamonds) plants is given.