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. 2000 Mar 28;97(8):4029–4033. doi: 10.1073/pnas.040561197

Figure 1.

Figure 1

(A) Structure of the region of the chromosomal copy of clag9 used for production of the vector pAC4-Clag9. Arrows indicate primer locations. (▧) Region of clag9 gene incorporated into the vector pAC4-Clag9. ( Created by potrace 1.16, written by Peter Selinger 2001-2019 ) Region of clag9 gene 5′ and 3′ to that incorporated into the vector pAC4-Clag9. (B) Structure of the knockout construct pAC4-CLAG. Large arrows indicate the plasmid backbone; small arrows indicate primer locations. (▤) P. falciparum calmodulin 5′ untranslated region. (Inline graphic) Toxoplasma gondi dihydrofolate reductase, thymidylate synthase, gene. (▥) P. falciparum histidine-rich protein two 3′ untranslated region. (C) PCRs testing for the presence of vector integrated into the chromosomal copy of clag9. Lanes 1–5, 3D7 DNA; lanes 6–10, clag9 transfectant DNA 19 weeks after transfection. Lanes 1 and 6, primers for the KAHRP gene. Lanes 2 and 7, primers Cso1 and S2EB (test for uninterrupted clag9). Lanes 3 and 8, primers Cso1 and DCH42 (integration test). Lanes 4 and 9, primers LAV2 and S2EB (integration test). Lanes 5 and 10, negative controls.