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The Gas1p maturation delay of Δemp24 cells is not exacerbated in p24Δ4 and p24Δ8 cells. Cells were pulse labeled for 5 min and chased for the indicated times. Gas1p was immunoprecipitated from cell extracts, resolved by SDS–PAGE, and detected by autoradiography. Migration positions of mature (125-kDa) and immature (105-kDa) forms are indicated.
