Figure 6.

Retrieval of Suc2-Wbp1p-KKTN (KK) and Suc2-Wbp1p-QKTN (QK) in wild-type, mutant, and p24 deletion strains. Cells were pulse labeled for 10 min and chased for 0 or 1 h. The fusion protein was immunoprecipitated from cell extracts, treated with endoglycosidase H, resolved by SDS–PAGE, and detected by autoradiography. Migration positions of immature and processed (vacuole) forms are indicated. Percentages of processed forms are averages of three experiments with standard deviations where applicable. The asterisk denotes a band that may represent either an intermediate PEP4-independent proteolytic product or nonspecific cross-reactive material, which was not included in the quantitation.