Figure 5.

Dose and time dependencies of PD173074-induced apoptosis in different human pancreatic cancer cell lines. Cells were treated with PD173074 (10 µM) for the indicated time (A) or cultured for 48 hours in the presence of the indicated doses of PD173074 (B). Cells were trypsinized and stained with annexin V and PI. Annexin V-positive and PI-negative cells were considered apoptotic. Values represent the mean ± SE of at least three independent experiments. *P < .05 indicates that for all cell lines studied, values for PD173074-treated cells were statistically significantly greater than those for untreated cells. ns = not significant.