Table 1.

Cell Cycle Phase Distribution of Pancreatic Cancer Cells Treated with PD173074.

		AsPC-1 (%)		Capan-1 (%)		HPAF-II (%)		MIA PaCa-2 (%)		PANC-1 (%)
		Control	PD173074	Control	PD173074	Control	PD173074	Control	PD173074	Control	PD173074
48 hr	G0/G1	39	59	42	59	46	55	41	58	39	67
	S	43	32	37	29	35	29	48	31	34	18
	G2/M	18	9	21	12	19	15	11	11	27	15
96 hr	G0/G1	43	69	39	64	47	61	38	76	42	77
	S	49	25	42	21	33	22	45	9	35	11
	G2/M	8	6	19	15	20	17	17	15	23	12

Logarithmically growing pancreatic cancer cells were collected after 48 and 96 hours of treatment with 10 µM PD173074 and stained with PI, and DNA content was subjected to flow cytometry analysis. The percentage of cells in each cell cycle phase was determined by the analysis of DNA content histograms using ModFit LT software. Numbers in the G₀/G₁ phase that are presented in italics are statistically different from the corresponding control values (P < .05, Student's t test). Values from a representative experiment are presented, and similar results were obtained in at least two independent experiments.