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In vitro processing of pre-tRNATyrII with an RNase Z-active fraction. The RNase Z-containing flow-through fraction from the Bio-Rex 70 column (a cation exchanger) was incubated with the pre-tRNATyrII substrate. A size marker (DNA) is indicated at left, sizes in nucleotides. Precursor (238 nt) and products (tRNA, 83 nt; 3′ trailer, 155 nt) are shown schematically at the right.
