Abstract
The microbiological bioassay, the adenylation method, and the radiometric, enzyme, and fluorescent immunoassay methods for assaying serum gentamicin were compared. The precision, reproducibility, and specificity of each method was assessed and proved satisfactory, with the exception of the radioimmunoassay, which gave artificially high results. Good correlations between the other four methods was obtained in a comparison involving 103 patient sera. The other aspects of performance were also compared, namely, simplicity, speed, cost, ease of automation, and application to large and small workloads. The enzyme immunoassay performed best in this comparison, being accurate, specific, rapid, and very simple to perform. However, other laboratories might find that workload, staffing, and available equipment make other methods more attractive.
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Selected References
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- Benveniste R., Davies J. R-factor mediated gentamicin resistance: A new enzyme which modifies aminoglycoside antibiotics. FEBS Lett. 1971 May 20;14(5):293–296. doi: 10.1016/0014-5793(71)80282-x. [DOI] [PubMed] [Google Scholar]
- Burd J. F., Wong R. C., Feeney J. E., Carrico R. J., Boguslaski R. C. Homogeneous reactant-labeled fluorescent immunoassay for therapeutic drugs exemplified by gentamicin determination in human serum. Clin Chem. 1977 Aug;23(8):1402–1408. [PubMed] [Google Scholar]
- Burnett G. H., Conklin R. L., Wasson G. W., MacKinney A. A. Variability of standard curves in radioimmunoassay of plasma digoxin. Clin Chem. 1973 Jul;19(7):725–726. [PubMed] [Google Scholar]
- Nossal N. G., Heppel L. A. The release of enzymes by osmotic shock from Escherichia coli in exponential phase. J Biol Chem. 1966 Jul 10;241(13):3055–3062. [PubMed] [Google Scholar]
- O'Leary T. D., Ratcliff R. M., Geary T. D. Evaluation of an enzyme immunoassay for serum gentamicin. Antimicrob Agents Chemother. 1980 May;17(5):776–778. doi: 10.1128/aac.17.5.776. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reeves D. S., Bywater M. J. Quality control of serum gentamicin assays--experience of national surveys. J Antimicrob Chemother. 1975 Mar;1(1):103–116. doi: 10.1093/jac/1.1.103. [DOI] [PubMed] [Google Scholar]
- Smith D. H., Van Otto B., Smith A. L. A rapid chemical assay for gentamicin. N Engl J Med. 1972 Mar 16;286(11):583–586. doi: 10.1056/NEJM197203162861106. [DOI] [PubMed] [Google Scholar]
- Stevens P., Young L. S., Hewitt W. L. Radioimmunoassay, acetylating radio-enzymatic assay, and microbioassay of gentamicin: a comparative study. J Lab Clin Med. 1975 Aug;86(2):349–359. [PubMed] [Google Scholar]
- White L. O., Scammell L. M., Reeves D. S. An evaluation of a gentamicin fluoroimmunoassay kit; correlation with radioimmunoassay, acetyl transferase and microbiological assay. J Antimicrob Chemother. 1980 Mar;6(2):267–273. doi: 10.1093/jac/6.2.267. [DOI] [PubMed] [Google Scholar]