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. 2000 Mar 28;97(8):4058–4063. doi: 10.1073/pnas.070526297

Figure 2.

Figure 2

Stra13 negatively regulates its own expression. (A) COS-7 cells were transfected with 0.5 μg of Stra13 promoter constructs pGL3KN and pGL3PmN. Forty-eight hours after transfection, luciferase activity was measured by using pGL3 as a control. The activity of pGL3PmN was plotted relative to pGL3KN, which was given an arbitrary value of 100. The abbreviations for the restriction enzyme sites used to generate the constructs are: K, KpnI; Pm, PmlI; and N, NheI. (B) COS-7 cells were transfected with the reporter pGL3KN in the presence of increasing amounts of a Stra13 expression vector (1–411). The extent of repression in the presence of Stra13 was measured relative to the activity of pGL3KN in the presence of equivalent amounts of the empty vector (pCS2) alone. (C) pGL3KN reporter (0.5 μg) was cotransfected into COS-7 cells with varying amounts of Stra13 expression vector or equivalent concentrations of an empty vector (pCS2), as indicated. TSA (100 nM) was added 24 h after transfection, and the cells were harvested 20 h later. The luciferase activity of pGL3KN in the absence or presence of TSA was given a value of 100. Note, however, that treatment with TSA alone resulted in a 2- to 3-fold increase in the promoter activity of pGL3KN.