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. 2000 Mar 28;97(8):4058–4063. doi: 10.1073/pnas.070526297

Figure 4.

Figure 4

Stra13-mediated repression requires the C-terminal α-helices. (A) COS-7 cells were transfected with 0.25 μg of the Stra13 reporter constructs pGL3KN or pGL3PmN and the same amount of Stra13 expression vectors Stra13 (1–411) or Stra13 (1–127). Luciferase activity was measured 48 h after transfection, and the activity of pGL3PmN was expressed relative to pGL3KN, which was given an arbitrary value of 100. (B) COS-7 cells were transfected with His-Stra13 (1–127). Forty-eight hours after transfection, cells were fixed and stained with anti-His mAb (Qiagen).