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. 2000 Mar 28;97(8):4058–4063. doi: 10.1073/pnas.070526297

Figure 5.

Figure 5

Stra13 negatively regulates c-Myc expression. (A) The c-Myc promoter was transfected in COS-7 cells with increasing concentrations of a Stra13 expression vector. Cells were harvested 48 h after transfection and assayed for luciferase activity. (B) The c-Myc promoter construct was cotransfected with Stra13 expression vector in COS-7 cells as described in A in the absence or presence of 100 nM TSA. The luciferase activity in the absence and presence of TSA was given a value of 100. (C) COS-7 cells were transfected with c-Myc promoter construct in the absence or presence of a Stra13 expression vector as well as a TFIIB expression vector. Cell lysates were assayed for luciferase activity after 48 h. (D) COS-7 cells were transfected with pGL3KN in the absence or presence of a Stra13 and TFIIB expression vector as indicated. Cell extracts were assayed for luciferase activity 48 h after transfection.