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. 2000 Mar 28;97(8):4070–4075. doi: 10.1073/pnas.080064097

Figure 1.

Figure 1

CaMK and MAPK target different domains of MEF2. Primary neonatal rat cardiomyocytes in serum-free medium were transiently transfected with the indicated expression plasmids, and luciferase activity was determined in cell extracts. (A) Cells were stimulated with PE (10 μM) or 10% FBS, as indicated, and expression of the MEF2-dependent reporter, 3xMEF2-luciferase, was assayed. (B) Cells transiently transfected with pG5E1b-luciferase (Gal-luc) and GAL-MEF2C were stimulated with PE, as in A, in the presence of KN62 or SB202190, as indicated. (C) Cells were transiently transfected with pG5E1b-luciferase and GAL-MEF2C (Left) or GAL-MEF2C-ΔN (Right), along with activated CaMKIV and MKK6, as indicated. A schematic of the GAL4-MEF2 fusions is shown at the bottom.