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. 2007 Feb 13;104(8):2861–2866. doi: 10.1073/pnas.0611487104

Fig. 3.

Fig. 3.

Fas and FasL are expressed in islets and regulate insulin and PDX-1 mRNA expression. (A and B) Shown is RT-PCR analysis of FasL (A) and Fas (B) expression in wild-type and Fas-deficient islets. Each lane represents an individual animal. GAPDH was used as control. (C–F) Fas-deficient and wild-type islets were transfected with a vector encoding for Fas and incubated for 8 days. (C) RT-PCR of Fas and GAPDH (control) expression in Fas-deficient and wild-type islets. (D) Immunostaining for Fas and insulin. (E and F) Quantitative RT-PCR detection of insulin (E) and PDX-1 (F) mRNA expression normalized to GAPDH (n = 3 for each group). ∗, P < 0.05 relative to wild type; #, P < 0.05 relative to Fas-deficient control.