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. 2007 Feb 13;104(8):2867–2872. doi: 10.1073/pnas.0611493104

Fig. 3.

Fig. 3.

Coimmunoprecipitation of EPCR and PAR-1 and immunoblotting of lipid rafts derived from EA.hy926 cells with anti-PAR-1, anti-EPCR, and anti-TM antibodies. (A) Immunoblots of total cellular proteins immunoprecipitated with either anti-EPCR or anti-PAR-1 antibody. (B) SDS/PAGE and immunoblotting of membrane fractions prepared by discontinuous sucrose gradient ultracentrifugation with antibodies directed to PAR-1, EPCR, TM, and decay-accelerating factor (DAF). (C) The same as in B except that the fractions were derived from EA.hy926 cells treated with 10 mM MβCD.