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. 2007 Feb 13;104(8):2867–2872. doi: 10.1073/pnas.0611493104

Fig. 4.

Fig. 4.

Effect of different concentrations of MβCD on the protective activity of APC in thrombin-induced permeability assay and on the PAR-1 cleavage rate in cells transfected with the PAR-1 cleavage reporter. (A) EA.hy926 cells were treated with the indicated concentrations of MβCD before evaluating the barrier protective effect of APC (○) in a thrombin-induced permeability assay. Controls are cells treated with thrombin only (●) and cells not treated with either thrombin or APC (■). (B) The rate of PAR-1 cleavage by APC (100 and 200 nM) monitored by ALP activity on EA.hy926 cells transiently transfected with the PAR-1 cleavage reporter at different concentrations of MβCD (1–10 mM) as described in the legend of Fig. 1.