Skip to main content
. 2007 Feb 14;104(8):2897–2902. doi: 10.1073/pnas.0608213104

Fig. 4.

Fig. 4.

GTPase activation and actin remodeling by PLY are cholesterol-dependent. (A) Cholesterol pretreatment of the toxin led to a partial (1:1 toxin:cholesterol wt/wt ratio) or full (1:5 ratio) inhibition of the actin changes 12 min after 0.1 μg/ml PLY treatment. Chol, cholesterol. (Scale bars, 10 μm.) (B) Cholesterol pretreatment of the toxin (1:5 toxin:cholesterol ratio) and cell membrane cholesterol depletion with 2 mM MβCD before 0.1 μg/ml PLY challenge for 12 min inhibited the cellular toxin binding. (C) Membrane cholesterol depletion with 2 mM MβCD fully inhibited the activation of RhoA and Rac1 after 8 min of 0.1 μg/ml PLY treatment.