Fig. 2.

Specificity and selectivity of p65 cleavage by Chlamydia. (A) HeLa 229 cells were infected with LGV2 at various multiplicities of infection for 28 h. The expression of p50/105, IκBε, and IκBα, molecules of the NF-κB signaling pathway, in infected and uninfected control cells was determined by blotting analysis. Erk2 expression was used as a loading control. (B and C) The cleavage of p65 protein is species-specific. Monolayers of human and mouse cells were infected with LGV2 for 28 h. The expression and cleavage of p65 was detected by immunoblot analysis. No p65 cleavage was detected from Chlamydia-infected murine cells. MOMP content in murine cells was determined as an indication of productive infection. +, A sample of Chlamydia-infected 293 cell lysate as a positive control.