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. 2007 Feb 13;104(8):2973–2978. doi: 10.1073/pnas.0605770104

Fig. 7.

Fig. 7.

bFGF blocks proliferation of medulloblastoma cells from ptc mutant mice. (A and B) FGF inhibits Shh target genes and proliferation. Tumor cells from ptc+/− mice were pretreated with DMSO or FGFR inhibitor (PD173074, 100 nM), cultured in the absence (Control) or presence of bFGF for 24–48 h, and then harvested for RNA analysis (A) or pulsed and assayed for 3H-Td incorporation (B). Data represent means ± SEM of six samples. ∗, P < 0.01; ∗∗, P < 0.001 (one-way ANOVA). (C–H) FGF promotes differentiation of tumor cells. Tumor cells from Math1-GFP/ptc+/− mice were cultured with no stimulus (Con) (C, E, and G) or bFGF (D, F, and H) for 24–48 h and then photographed by using bright-field or fluorescent microscopy. (C and D) Bright-field images at 24 h. (E and F) Math1-GFP expression at 24 h. (G and H) Math1-GFP expression at 48 h. (Magnification: ×20).