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. 2000 Apr 11;97(8):4076–4081. doi: 10.1073/pnas.97.8.4076

Figure 3.

Figure 3

A dominant inhibitory GATA-binding protein represses endoderm development in Xenopus embryos. (A) Design of the dominant-inhibitory construct End-1∷EnR fusing the putative DNA-binding domain of END-1 (aa 106–221) to the transcriptional repression domain (aa 2–298) of Drosophila Engrailed. (B) Phenotype of Xenopus embryos at stage 42/43 after microinjection of 100 pg of End-1∷EnR or control (End-1Δ3′) mRNA into the vegetal hemisphere at the one-cell stage. (C) Rescue of lethal developmental effects of vegetal expression of End-1∷EnR by coinjection of wild-type End-1. Two-cell embryos were injected in the vegetal hemisphere of each blastomere with 400 pg of Sox17β∷EnR or End-1∷EnR mRNA, and 400 pg of either End-1 (panel 3) or End-1Δ3′ (panels 1 and 2) mRNA. (D) Down-regulation of mature markers of Xenopus endoderm in vegetal explants treated with End-1∷EnR. Two-cell embryos were injected as above with 250 pg of Sox17β∷EnR or End-1∷EnR mRNA and 250 pg of End-1 (lane 6) or End-1Δ3′ (lanes 3–5) mRNA. Vegetal explants were cultured until the equivalent of Stage 35. RT-PCR for ODC serves as a control for equal RNA input.