Figure 5.

Placing GATA activity within the context of Mixer/Mix.3 and Sox17 functions in Xenopus endoderm development. One-cell embryos were injected with 200 pg of endoderm-inducing mRNAs, and 400 pg of either mRNA-encoding dominant-inhibitory EnR fusion proteins or EF-1α “filler” mRNA. Animal cap explants were cultured and RNA analyzed by RT-PCR for Sox17α when sister tadpoles reached Nieuwkoop-Faber Stage 15 (A), for endodermin (Edd) at the equivalent of Stage 40 (B), and for ODC as a loading control.