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. Author manuscript; available in PMC: 2008 Feb 1.
Published in final edited form as: Exp Eye Res. 2006 Nov 30;84(2):361–372. doi: 10.1016/j.exer.2006.10.011

Fig. 4.

Fig. 4

Time-dependent effects of hypertonicity on SAPK/JNK activity. RCEC and HCEC were exposed to 600 mOsm medium for up to 6 h. SAPK/JNK was pulled down by the N-terminal portion of the c-Jun transcription factor, and its activity evaluated based on its ability to phosphorylate c-Jun. Inset: Representative Western blot of typical experiments performed on “pull-downs” obtained from HCEC (inset A) and RCEC (inset B) lysates. Data are presented as the mean ±SEM of independent experiments carried out in triplicate for each osmolarity.